Part:BBa_K4090007

csgA-Mfp5-Mfp5

A kind of fusion protein that helps to form hydroxycalcium phosphate outside the membrane. Since the expression of a single Mfp5 gene is insufficient to cause a notable precipitation, our team transformed two mfp5 sequences into the plasmid.

Fig.1

Fig.2

Data

Fig.3

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 371
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 371
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 556
  Illegal BamHI site found at 784
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 371
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 371
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1] DHAMI N K, REDDY M S, MUKHERJEE A. Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites[J]. Journal of Microbiology and Biotechnology, 2013, 23(5): 707-714.
[2] OKWADHA G D, LI J. Optimum conditions for microbial carbonate precipitation[J]. Chemosphere, 2010, 81(9): 1143-1148. [16]LI W, LIU L P, ZHOU P P, et al. Calcite precipitation induced by bacteria and bacterially produced carbonic anhydrase[J]. Current Science, 2011，100(4): 502-508.
[3] ANBU P, KANG C H, SHIN Y J, et al. Formations of calcium carbonate minerals by bacteria and its multiple applications[J]. Springerplus, 2016, 5(1): 1-26.
[4] Hale, L. V., Ma, Y. F. & Santerre, R. F. Semi-quantitative fuorescence analysis of calcein binding as a measurement of in vitro mineralization. Calcif. Tissue Int. 67, 80-84(2000). Ramachandran S K.
[5] Dick J, Windt W D, Graef B D, et al. Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species[J]. Biodegradation, 2016(4): 357-367.
[6] Qian C X, Chen H C, Ren L F, et al. Self-healing of early age cracks in cement-based materials by mineralization of carbonic anhydrase microorganism[J]. Frontiers in Microbiology, 2015(6): 1-9.