![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3771020
Designed by: KAO, YI-CHING Group: iGEM21_NCKU_Tainan (2021-10-01)
PpspA-JJU
Description
This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, JJU10.
Biology
Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [3] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of JJU10. JJU10 converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.
Usage
We ligased the JJU10 fragment and pspA promoter on the pSU expression vector and transformed it into DH5alpha to complete construction of the plasmid.
Characterization
![](https://2021.igem.org/wiki/images/e/e0/T--NCKU_Tainan--colony_pcr_pspA_JJU10.jpg
)
圖片描述
Sequence and Features
[edit]
Categories
Parameters
None |