Regulatory

Part:BBa_K3771047

Designed by: KAO, YI-CHING   Group: iGEM21_NCKU_Tainan   (2021-10-01)
Revision as of 10:51, 17 October 2021 by Karin100699 (Talk | contribs)


PkatG


Description


PkatG is the promoter of the katG gene. KatG protein has the function of bifunctional hydroperoxidase I (HPI) and plays an important role in oxidative stress response. The katG promoter (PkatG) is stimulated by oxidative stress, leading to the expression of the downstream gene. Besides, PkatG is activated by OxyR protein[1].

Figure 1. Transcription unit of the katG gene in E. coli K-12[2].



Usage and Biology


After conducting colony PCR from the E. coli MG1655, the PkatG fragment can be amplified from the chromosome and the experiment result can be checked by agarose gel electrophoresis. The result is shown in Figure 2.

Figure 2. The electrophoresis result of PkatG fragment from the chromosome PCR. M: Marker; Lane 1: katG promoter (PkatG) (217 bp).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None