Regulatory

Part:BBa_J70301:Design

Designed by: Tom Knight   Group: Knight Lab   (2009-03-29)
Revision as of 20:39, 6 April 2009 by Tk (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Heat shock inducible promoter for Me. florum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Low gc content, difficult PCR primers


primers:


GTTTCTTC GAATTC GCGGCCGC T TCTAGA G caaataacggataattataaaataaaac,J70301-F

GTTTCTTC CTGCAG CGGCCGC T ACTAGT A ctatgtggttaaatatatattatcatattg,J70301-R


474647 - 474537 (reverse orientation) from NC_006055

Source

PCR from lon protease promoter of NC_006055.

References