Coding

Part:BBa_K3784001

Designed by: LUXUAN LIU   Group: iGEM21_BNUZ-China   (2021-09-19)
Revision as of 09:38, 17 October 2021 by MANUL (Talk | contribs)


fldB (Encoding 2-hydroxyacyl-CoA dehydratase protein family)

The fldB is from the FLD gene cluster of Clostridium sporogenes, involved in the conversion of tryptophan to 3-Indolepropionic acid. In the five-step metabolic pathway we reconstructed, fldB encodes encodes subunit of 2-hydroxyacyl-CoA dehydratase protein, combined with another subunit encoded by fldC to become 2-hydroxyacyl-CoA dehydratase protein, which catalyze indole lactic acid to produce indole acrylic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
    Illegal BglII site found at 48
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None