Composite

Part:BBa_K3771013

Designed by: KAO, YI-CHING   Group: iGEM21_NCKU_Tainan   (2021-10-01)
Revision as of 09:33, 17 October 2021 by Evelynnn (Talk | contribs)


PlacI-OFP


Description


PlacI-OFP is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.


Biology


Without the presence of the LacI repressor, the LacI promoter facilitates the constitutive expression of OFP.


Characterization

<img src="T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;">

Double digestion results are shown in Fig. 2.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None