Coding

Part:BBa_K3815002

Designed by: Shiotsu Koudai   Group: iGEM21_Kyoto   (2021-10-15)
Revision as of 09:23, 17 October 2021 by Shiotsu (Talk | contribs)


Defensin1-Mxe GryA intein-PTlinker-ELK16

This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein. In this part, the targeted protein is Defencine1 derived from a honey bee. This is antimicrobial peptide that can inhibit the growth of gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 249
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
    Illegal NgoMIV site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None