Coding
Part:BBa_K3771010
Designed by: KAO, YI-CHING Group: iGEM21_NCKU_Tainan (2021-10-01)
Revision as of 07:47, 17 October 2021 by Jason Tsai (Talk | contribs)
Outer Membrane Protein A (OmpA)
Description
Outer membrane protein A (OmpA) is a 35 kDa transmembrane protein found in many enterobacteria. It is responsible for maintaining the stability of the bacterial membrane and cell signaling. [1]
Biology
In E. coli, the β-barrel conformation of OmpA is composed of extracellular loops that help play a role in the detection of outer membrane stress and binding of extracellular molecules. OmpA also serves as a porin channel that regulates water transport within the bacterial cell. [2]
Usage
In our project, the sequence of OmpA protein was used to construct the OmpA/OprF chimeric protein.
<figure class="d-flex flex-column justify-content-center align-items-center px-lg-3"> <a href="https://2021.igem.org/File:T--NCKU_Tainan--ompA_oprF.gif" target="_blank" style="width:60%"><img src="https://2021.igem.org/File:T--NCKU_Tainan--ompA_oprF.gif" alt="" title="" style="width:60%"></a> </figure>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 881
Illegal BamHI site found at 744 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |