Coding

Part:BBa_K3771004

Designed by: KAO, YI-CHING   Group: iGEM21_NCKU_Tainan   (2021-09-30)
Revision as of 06:27, 17 October 2021 by ShaneLee (Talk | contribs)


CDO1


Description
CDO1 is part of the L-cysteine sulfinic acid pathway, one of three possible taurine synthesis pathways. CDO1 oxidizes L-cysteine naturally found in the intestine into L-cysteine sulfinic acid, which is later converted to taurine by CSAD.[1]

<img src="T--NCKU_Tainan--taurine_pathway_1.png" style="width:35%;">


Usage
CDO1 was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 447
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None