Coding

Part:BBa_K3729002

Designed by: Austin Wang, Julie Lin, Sinswien Lee   Group: iGEM21_KCIS_NewTaipei   (2021-10-09)
Revision as of 05:30, 17 October 2021 by JulieLin (Talk | contribs)


BCD 1: (Acyl-coa Dehydrogenase)

Beta-hydroxybutyryl-CoA dehydrogenase catalyzes an important step (acetyl-CoA to 3-hydroxybutyryl-CoA) in the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (HBD) encoded for butyryl- CoA dehydrogenase from the genome of E. coli Nissle 1917.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 438
    Illegal BglII site found at 1056
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 197


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Categories
Parameters
None