Coding
Part:BBa_K3729001
Designed by: Austin Wang Group: iGEM21_KCIS_NewTaipei (2021-10-08)
BCD 1: (Acyl-coa Dehydrogenase)
Butyryl- CoA dehydrogenase catalyzes the rate-limiting step (Crotonyl-CoA to butyryl-CoA) of the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (BCD) encoded for butyryl- CoA dehydrogenase from the genome of Clostridium acetobutylicum.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 438
Illegal BglII site found at 1056 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 224
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 197
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Categories
Parameters
None |