Part:BBa_K3740009
Holin S
Description
Lysis of the engineered bacteria
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
2021 SZPT-China
Biology
The S gene of lambda bacteriophage has the characteristics of a double initiation motif,which can encode two proteins with opposite functions,S107 and S105. Among them,S105 plays the function of perforin,S107 also participates in the formation of transmembrane pores when triggering lysis,but before triggering lysis,it has the function of antagonizing S105 to prevent the premature formation of transmembrane pores,so S107 is called anti-perforin. The interaction between S105 and S107 is inseparable,collectively referred to as S protein. The structure directed initiation (sdi)of the S gene to form or open the stem-loop structure at the ribosome binding site (RBS)sequence GGGGG encoding S107,thereby regulating the synthesis ratio of the two proteins S107 and S105.
References
[1] Yibo S, Jianhe S, Shi Y, et al. Current advance in the topological structure and function of holin encoded by bacteriophage lambda[J]. Acta Microbiologica Sinica, 2012, 52(2):141-145.
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