Part:BBa_K3766013

mCherry (reverse orientation)

This codon optimized mCherry for Escherichia coli K12 is the BBa_K2448004 in reverse orientation.

Usage and Biology

mCherry [1] is a fluorescent monomeric protein widely used in biotechnology. Like the majority of red fluorescent proteins, it is derived from the Red Fluorescent protein from Discosoma sp. mCherry is sometimes preferred over other fluorophores due to its color, its rapid maturation time (t0.5=15 min), its lower brightness (around 50% of eGFP) as well as its improved photostability and resistance to bleaching.

References

[1] Shaner NC, Campbell RE, Steinbach PA, Giepmans BNG, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology (2004) 22: 1567–1572.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 25
  Illegal AgeI site found at 137
  
• 1000
  COMPATIBLE WITH RFC[1000]