Composite

Part:BBa_K3868126

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 17:39, 16 October 2021 by Registry (Talk | contribs)

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pYLXP- 17-Nluc


pYLXP- 17-Nluc plasmid contains pYLXP-17, Nluc etc. The recombinant plasmid of pYLXP-17-Nluc was constructed using linearized pYLXP-PTEF-Nluc (digested by AvrII and NheI) and the PCR-amplified promoter pYLXP- 17 sequences by Gibson Assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 651
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 776
    Illegal XhoI site found at 41
    Illegal XhoI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1545
    Illegal AgeI site found at 749
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 83


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