Composite
Part:BBa_K3868123
Designed by: Yan Xu Group: iGEM21_NNU-China (2021-10-16)
pYLXP- 14-Nluc
pYLXP- 14-Nluc plasmid contains pYLXP-14, Nluc etc. The recombinant plasmid of pYLXP-14-Nluc was constructed using linearized pYLXP-PTEF-Nluc (digested by AvrII and NheI) and the PCR-amplified promoter pYLXP- 14 sequences by Gibson Assembly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 281
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1552
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1246
Illegal SapI site found at 103
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Categories
Parameters
None |