Composite

Part:BBa_K3868122

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 17:33, 16 October 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pYLXP- 13-Nluc


pYLXP- 13-Nluc plasmid contains pYLXP-13, Nluc etc. The recombinant plasmid of pYLXP-13-Nluc was constructed using linearized pYLXP-PTEF-Nluc (digested by AvrII and NheI) and the PCR-amplified promoter pYLXP- 13 sequences by Gibson Assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 731
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 731
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 731
    Illegal BamHI site found at 896
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 731
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 731
    Illegal NgoMIV site found at 1552
    Illegal AgeI site found at 46
    Illegal AgeI site found at 103
    Illegal AgeI site found at 410
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None