![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3868122
Designed by: Yan Xu Group: iGEM21_NNU-China (2021-10-16)
pYLXP- 13-Nluc
pYLXP- 13-Nluc plasmid contains pYLXP-13, Nluc etc. The recombinant plasmid of pYLXP-13-Nluc was constructed using linearized pYLXP-PTEF-Nluc (digested by AvrII and NheI) and the PCR-amplified promoter pYLXP- 13 sequences by Gibson Assembly.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 731
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 731
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 731
Illegal BamHI site found at 896 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 731
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 731
Illegal NgoMIV site found at 1552
Illegal AgeI site found at 46
Illegal AgeI site found at 103
Illegal AgeI site found at 410 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |