Regulatory

Part:BBa_J70301:Design

Designed by: Tom Knight   Group: Knight Lab   (2009-03-29)
Revision as of 21:19, 29 March 2009 by Tk (Talk | contribs) (→‎Design Notes)

Heat shock inducible promoter for Me. florum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Low gc content, difficult PCR primers


primers:


GTTTCTTC GAATTC GCGGCCGC T TCTAGA G caaataacggataattataaaataaaac

GTTTCTTC CTGCAG CGGCCGC T ACTAGT A ctatgtggttaaatatatattatcatattg

Source

PCR from lon protease promoter of NC_006055.

References