Composite
Part:BBa_K3868118
Designed by: Yan Xu Group: iGEM21_NNU-China (2021-10-16)
pYLXP- 9-Nluc
pYLXP- 9-Nluc plasmid contains pYLXP-9, Nluc etc. The recombinant plasmid of pYLXP-9-Nluc was constructed using linearized pYLXP-PTEF-Nluc (digested by AvrII and NheI) and the PCR-amplified promoter pYLXP- 9 sequences by Gibson Assembly.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1319
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1319
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1181
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1319
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1319
Illegal NgoMIV site found at 1552 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |