Composite
Part:BBa_K3868115
Designed by: Yan Xu Group: iGEM21_NNU-China (2021-10-16)
pYLXP- 6-Nluc
pYLXP- 6-Nluc plasmid contains pYLXP-6, Nluc etc. The recombinant plasmid of pYLXP-6-Nluc was constructed using linearized pYLXP-PTEF-Nluc (digested by AvrII and NheI) and the PCR-amplified promoter pYLXP- 6 sequences by Gibson Assembly.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1444
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 6
Illegal SpeI site found at 1444 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1444
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1444
Illegal NgoMIV site found at 1552 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |