Composite

Part:BBa_K3868115

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 17:25, 16 October 2021 by Registry (Talk | contribs)

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pYLXP- 6-Nluc


pYLXP- 6-Nluc plasmid contains pYLXP-6, Nluc etc. The recombinant plasmid of pYLXP-6-Nluc was constructed using linearized pYLXP-PTEF-Nluc (digested by AvrII and NheI) and the PCR-amplified promoter pYLXP- 6 sequences by Gibson Assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1444
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6
    Illegal SpeI site found at 1444
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1444
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1444
    Illegal NgoMIV site found at 1552
  • 1000
    COMPATIBLE WITH RFC[1000]


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