Composite
Part:BBa_K3868114
Designed by: Yan Xu Group: iGEM21_NNU-China (2021-10-16)
pYLXP- 5-Nluc
pYLXP- 5-Nluc plasmid contains pYLXP-5, Nluc etc. The recombinant plasmid of pYLXP-5-Nluc was constructed using linearized pYLXP-PTEF-Nluc (digested by AvrII and NheI) and the PCR-amplified promoter pYLXP- 5 sequences by Gibson Assembly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 774
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1552
Illegal AgeI site found at 314 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 193
Illegal BsaI.rc site found at 881
Illegal SapI.rc site found at 840
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Categories
Parameters
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