Composite

Part:BBa_K3868114

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 17:23, 16 October 2021 by Registry (Talk | contribs)

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pYLXP- 5-Nluc


pYLXP- 5-Nluc plasmid contains pYLXP-5, Nluc etc. The recombinant plasmid of pYLXP-5-Nluc was constructed using linearized pYLXP-PTEF-Nluc (digested by AvrII and NheI) and the PCR-amplified promoter pYLXP- 5 sequences by Gibson Assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 774
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1552
    Illegal AgeI site found at 314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 193
    Illegal BsaI.rc site found at 881
    Illegal SapI.rc site found at 840


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