Composite

Part:BBa_K3868107

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 16:52, 16 October 2021 by Registry (Talk | contribs)

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pET-SPINK9-eGFP

pET-24a-SPINK9 plasmid contains pT7, lacO, SPINK9, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of SPINK9 was fused with eGFP in order to characterize the yield of SPINK9. Moreover, in order to purify the SPINK9, the enzyme loci of thrombin was inserted between SPINK9 and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-SPINK9-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of SPINK9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None