Composite

Part:BBa_K3868105

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 16:48, 16 October 2021 by Registry (Talk | contribs)

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pET-SMAP-29-eGFP

pET-24a-SMAP-29 plasmid contains pT7, lacO, SMAP-29, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of SMAP-29 was fused with eGFP in order to characterize the yield of SMAP-29. Moreover, in order to purify the SMAP-29, the enzyme loci of thrombin was inserted between SMAP-29 and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-SMAP-29-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of SMAP-29.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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