Composite
Part:BBa_K3868105
Designed by: Yan Xu Group: iGEM21_NNU-China (2021-10-16)
pET-SMAP-29-eGFP
pET-24a-SMAP-29 plasmid contains pT7, lacO, SMAP-29, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of SMAP-29 was fused with eGFP in order to characterize the yield of SMAP-29. Moreover, in order to purify the SMAP-29, the enzyme loci of thrombin was inserted between SMAP-29 and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-SMAP-29-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of SMAP-29.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |