Composite

Part:BBa_K3868104

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 16:45, 16 October 2021 by Registry (Talk | contribs)

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pET-LL-37-eGFP

pET-24a-LL-37 plasmid contains pT7, lacO, LL-37, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of LL-37 was fused with eGFP in order to characterize the yield of LL-37. Moreover, in order to purify the LL-37, the enzyme loci of thrombin was inserted between LL-37and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-LL-37-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of LL-37.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None