Composite

Part:BBa_K3868103

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 16:42, 16 October 2021 by Registry (Talk | contribs)

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pET-Oxysterlin 1-eGFP

pET-24a-Oxysterlin 1 plasmid contains pT7, lacO, Oxysterlin 1, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of Oxysterlin 1 was fused with eGFP in order to characterize the yield of Oxysterlin 1. Moreover, in order to purify the Oxysterlin 1, the enzyme loci of thrombin was inserted between and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-Oxysterlin 1-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of Oxysterlin 1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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