Composite
Part:BBa_K3868098
Designed by: Yan Xu Group: iGEM21_NNU-China (2021-10-16)
pTargetS
pTargetS plasmid contains pj23119, sgRNA-1 and sgRNA-2 etc.Two different sgRNA expression frames were tandemly linked, allowing GGGGGGGG to be covered, resulting in a more diverse editing outcome.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 182
Illegal EcoRI site found at 552
Illegal XbaI site found at 188
Illegal XbaI site found at 558
Illegal SpeI site found at 73
Illegal SpeI site found at 443
Illegal PstI site found at 200
Illegal PstI site found at 570 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 182
Illegal EcoRI site found at 552
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 50
Illegal NheI site found at 377
Illegal NheI site found at 400
Illegal NheI site found at 420
Illegal SpeI site found at 73
Illegal SpeI site found at 443
Illegal PstI site found at 200
Illegal PstI site found at 570 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 182
Illegal EcoRI site found at 552
Illegal BglII site found at 212
Illegal BglII site found at 582
Illegal XhoI site found at 236
Illegal XhoI site found at 606 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 182
Illegal EcoRI site found at 552
Illegal XbaI site found at 188
Illegal XbaI site found at 558
Illegal SpeI site found at 73
Illegal SpeI site found at 443
Illegal PstI site found at 200
Illegal PstI site found at 570 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 182
Illegal EcoRI site found at 552
Illegal XbaI site found at 188
Illegal XbaI site found at 558
Illegal SpeI site found at 73
Illegal SpeI site found at 443
Illegal PstI site found at 200
Illegal PstI site found at 570 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |