Coding

Part:BBa_K3989001:Design

Designed by: Song-Yuan Zhang   Group: iGEM21_UZurich   (2021-09-30)
Revision as of 15:59, 16 October 2021 by LHospital (Talk | contribs)


uTEV3, efficiency-enhanced TEV protease variant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sanchez, M. I., & Ting, A. Y. (2020). Directed evolution improves the catalytic efficiency of TEV protease. Nature methods, 17(2), 167-174.


Source

Original TEV protease sequence and structure. PDB: 1LVM

References

1) Denard, C. A., Paresi, C., Yaghi, R., McGinnis, N., Bennett, Z., Yi, L., ... & Iverson, B. L. (2021). YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants. ACS Synthetic Biology, 10(1), 63-71.