Part:BBa_K3989001

uTEV3, efficiency-enhanced TEV protease variant

uTEV3 is a mutant from the original TEV protease. Three amino acids(I138T, S153N, T180A) are mutated in this variant and the catalytic efficiency is higher than the original one and slightly lower than <a href="https://parts.igem.org/Part:BBa_K3989002"> BBa_K3989002</a>. This variant is generated by directed evolution in yeast.

Properties

The three mutations are at the binding site of the protease. The catalytic efficiency of this variant and the original TEV protease is shown in Fig.1.

Fig.1 Comparison of the catalytic efficiency between uTEV3 and the original

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

1) Sanchez, M. I., & Ting, A. Y. (2020). Directed evolution improves the catalytic efficiency of TEV protease. Nature methods, 17(2), 167-174.