Composite

Part:BBa_K3753014:Design

Designed by: Yiqing Zhang   Group: iGEM21_NJTech_China   (2021-10-16)
Revision as of 15:32, 16 October 2021 by RI2020 (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pTEF2-GFP-CYC1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 490
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1644


Design Notes

The promoter of tef2 was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The green fluorescent protein and the terminator CYC1(GFP-CYC1) was obtained by PCR amplification using pRS415-GFP-CYC1 plasmid as template.

Source

Saccharomyces cerevisiae

References