Regulatory

Part:BBa_K3753003

Designed by: Jiaying Hu   Group: iGEM21_NJTech_China   (2021-10-13)
Revision as of 15:27, 16 October 2021 by HJY (Talk | contribs) (→‎Characterization)

pTEF2

pTEF2 is a strong constitutive promoter in Saccharomyces cerevisiae, which controls the transcription of its downstream sequence coding for translational elongation factor EF-1 α.

Characterization


Fig.1 The fluorescence intensity of GFP expressed by engineered promoters


When assembled with GFP BBa_K3112009, and CYC1 terminator BBa_K3384311 in pRS426, pTEF2 regulated the expression of GFP. Three pTEF2-based synthetic hybrid promoters were designed to enhance promoter activity. For detailed information, please visit UASCLB-pTEF BBa_K3753011, UASCIT-pTEF BBa_K3753012, UASTEF-pTEF BBa_K3753013.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 490
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
chassisSaccharomyces cerevisiae