Regulatory

Part:BBa_K3753013:Design

Designed by: Yiqing Zhang   Group: iGEM21_NJTech_China   (2021-10-16)
Revision as of 14:15, 16 October 2021 by RI2020 (Talk | contribs) (→‎Design Notes)


UASTEF-pTEF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 629
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 629
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 629
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 629
    Illegal NgoMIV site found at 1151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 167
    Illegal BsaI.rc site found at 378
    Illegal BsaI.rc site found at 587


Design Notes

UASTEF-pTEF is synthesized by fusing three UASTEF tandem to the tef2 promoter (BBa_K3753003). UASTEF was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The amplified product is a 240 bp sequence 5' of the mitotic cyclin coding gene clb2.

Source

Saccharomyces cerevisiae

References