Regulatory

Part:BBa_K3753013

Designed by: Yiqing Zhang   Group: iGEM21_NJTech_China   (2021-10-16)
Revision as of 14:07, 16 October 2021 by Registry (Talk | contribs)

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UASTEF-pTEF

UASTEF-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from Translational elongation factor EF-1 α tef1, and the core promoter tef2. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 629
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 629
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 629
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 629
    Illegal NgoMIV site found at 1151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 167
    Illegal BsaI.rc site found at 378
    Illegal BsaI.rc site found at 587


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Parameters
chassisSaccharomyces cerevisiae