Composite
Part:BBa_K3868095
Designed by: Yan Xu Group: iGEM21_NNU-China (2021-10-16)
pCas
pCas plasmid of the Crispr-Cas9 system contains Pcas, Spcas9, paraB, λ-red, paraC and arac etc, which plays a role in cutting DNA double strands.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1572
Illegal EcoRI site found at 2469
Illegal EcoRI site found at 3978
Illegal PstI site found at 3473
Illegal PstI site found at 3720 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1572
Illegal EcoRI site found at 2469
Illegal EcoRI site found at 3978
Illegal NheI site found at 1331
Illegal PstI site found at 3473
Illegal PstI site found at 3720 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1572
Illegal EcoRI site found at 2469
Illegal EcoRI site found at 3978
Illegal BamHI site found at 2408 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1572
Illegal EcoRI site found at 2469
Illegal EcoRI site found at 3978
Illegal PstI site found at 3473
Illegal PstI site found at 3720 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1572
Illegal EcoRI site found at 2469
Illegal EcoRI site found at 3978
Illegal PstI site found at 3473
Illegal PstI site found at 3720
Illegal AgeI site found at 3900 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |