Regulatory

Part:BBa_K3753011:Design

Designed by: Yiqing Zhang   Group: iGEM21_NJTech_China   (2021-10-16)
Revision as of 13:04, 16 October 2021 by Registry (Talk | contribs)

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UASCLB-pTEF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 747
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 747
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 747
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 747
    Illegal NgoMIV site found at 1269
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

UASCLB-pTEF is synthesized by fusing three UASCLB tandem repeats to the pTEF native promoter ( BBa_K3753003). UASCLB was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The amplified product is a 240bp sequence upstream of the CLB2 start codon.


Source

Saccharomyces cerevisiae

References