Coding

Part:BBa_K3930008

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-07)
Revision as of 10:35, 16 October 2021 by Ambre-Jousselin (Talk | contribs)


Gene coding for the Linalool synthase Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1135
    Illegal PstI site found at 23
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1135
    Illegal PstI site found at 23
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1135
    Illegal PstI site found at 23
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1135
    Illegal PstI site found at 23
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

This sequence codes for the linalool synthase, which transform GPP into the flower-smelling compounds linalool, smelling like flower. The sequence derived from the Lavandula angustifolia genome. We take advantage of the publication of (Amiri et al. 2016).

There is already BBa_K3052002 on the registry for linalool synthase. This part is codon optimized for translation in E. coli, but our is codon optimized for translation in S. cerevisiae.


References

  1. Amiri P, Shahpiri A, Asadollahi MA, Momenbeik F, Partow S. 2016. Metabolic engineering of Saccharomyces cerevisiae for linalool production. Biotechnol Lett. 38(3):503–508. doi:10.1007/s10529-015-2000-4.


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