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Coding
davB
Part:BBa_K3776001
Designed by: Helene Heling Huang Group: iGEM21_HK_SSC (2021-10-01)
Revision as of 10:17, 16 October 2021 by Meowstarperson (Talk | contribs)
DavB
Designed by Vo et al. (2020), this protein complex includes transposases TnsA, TnsB, TnsC and Cas enzymes Cas 8, Cas7, Cas6. These proteins are guided by a crRNA prefix flanked by two repeats. Users should clone their crRNA between these two repeats. This RNA guided protein complex inserts a donor DNA located between two flanks behind the into the genome of the host organism. Users should clone their genes of interest between these two flanks.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 239
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1328
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1488
Illegal AgeI site found at 895
Illegal AgeI site found at 1144 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |