Coding
Part:BBa_K3815010
Designed by: Hiroto Koga Group: iGEM21_Kyoto (2021-10-16)
No part name specified with partinfo tag.
We used His tag to purify the peptides. The peptide His-tag fused was recovered by Ni chromatography, and DTT was added to cleave the N-terminus of the intein.Then, we can get the purpose protein. In this part, the purpose protein is NOP1v.This is a small peptide that can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene and aging of cut flowers. We could not get NOP1 sufficiently when using BBa_K3815038, so we added a valine to the N-terminus of NOP1 according to N end rule.
Sequence and Features No part name specified with partinfo tag.
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