Part:BBa_K3930030

Gene coding for the 9-lipoxygenase Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1406
  Illegal XhoI site found at 1442
  Illegal XhoI site found at 2425
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 342
  Illegal SapI site found at 1417
  

Introduction

This sequence codes for the 9-lipoxygenase which transforms linoleic and linolenic acids into their hydroperoxyde forms. This sequence is codon optimized for expression into S. elongatus. The 9-LOX sequence comes from Nicotiana benthamiana and is described in the publication of (Vincenti et al, 2019).

Testing

The Toulouse team could not test this part for reasons of time, and preferred to focus on the parts characterization that allow the production of molecules responsible for the floral aspect of the violet perfume.

References

1. Vincenti S, Mariani M, Alberti J-C, Jacopini S, Brunini-Bronzini de Caraffa V, Berti L, Maury J. 2019. Biocatalytic Synthesis of Natural Green Leaf Volatiles Using the Lipoxygenase Metabolic Pathway. Catalysts. 9(10):873. doi:10.3390/catal9100873.