Part:BBa_K3930031

Gene coding for the 9-Hydroperoxide lyase Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 18
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1012
  

Introduction

This sequence codes for the 9-Hydroperoxide lyase which transforms the hydroperoxyde forms of linoleic and linolenic acids into 3,6-nonadienal. This sequence is codon optimized for expression into S.elongatus. The 9-HPL sequence comes from Cucumis melo and is described in the publication of (Vincenti et al, 2019).

Testing

The Toulouse team could not test this part for reasons of time, and preferred to focus on the parts characterization that allow the production of molecules responsible for the floral aspect of the violet perfume.

References

1. Vincenti S, Mariani M, Alberti J-C, Jacopini S, Brunini-Bronzini de Caraffa V, Berti L, Maury J. 2019. Biocatalytic Synthesis of Natural Green Leaf Volatiles Using the Lipoxygenase Metabolic Pathway. Catalysts. 9(10):873. doi:10.3390/catal9100873.