Coding

Part:BBa_K3989002

Designed by: Tianyu Xu   Group: iGEM21_UZurich   (2021-09-30)
Revision as of 09:37, 16 October 2021 by LHospital (Talk | contribs) (→‎Structure and binding site)


eTEV, efficiency-enhanced TEV protease variant

eTEV protease is mutant from the original TEV protease. Seven amino acids(S3I, P8Q, S31T, E79G, T173A, V219R, A231V) are mutated in this variant and the catalytic efficiency is 2-fold higher than the original one.


Structure and binding site

<figure> 

 <img src="21_UZurich_eTEV_binding_site.jpeg">
 <figcaption>Fig. 1 . - Sub-structure at the binding site of eTEV. The blue amino acids are mutated and the purple one represents the cut site amino acid residue</figcaption>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

1) Denard, C. A., Paresi, C., Yaghi, R., McGinnis, N., Bennett, Z., Yi, L., ... & Iverson, B. L. (2021). YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants. ACS Synthetic Biology, 10(1), 63-71.

[edit]
Categories
Parameters
None