Coding

Part:BBa_K3868073

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-15)
Revision as of 07:46, 16 October 2021 by XSY3469 (Talk | contribs)


Nluc

Gene from S. pyogenes for the pCas plasmid of the Crispr-Cas9 system,plays a role in cutting DNA double strands.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pCas plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None