Composite

Part:BBa_K3796217

Designed by: Feiyue Yang   Group: iGEM21_CAU_China   (2021-10-01)
Revision as of 03:05, 16 October 2021 by Yang Feiyue (Talk | contribs)


Ptac-rbs-ndoA-terminator

This is the gene circuit that can be used to verify if the toxin gene, ndoA, works as expected. ndoA encodes endoribonuclease EndoA, a toxic component of a type II toxin-antitoxin (TA) system in Bacillus subtilis. We use this gene circuit to verify if ndoA is toxic for the growth of C. glutamicum as well, by comparing its growth state with/without induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None