RNA

Part:BBa_K4061041

Designed by: Diya Agrawal   Group: iGEM21_HKUST   (2021-10-15)
Revision as of 01:56, 16 October 2021 by Registry (Talk | contribs)

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Antinsense RNA against Constitutive eGFP

This is an antisense mRNA encoded to downregulate Constitutively expressed (BBa_J23100) eGFP fluorophore. This mRNA will be a part of the composite part (BBa_K4061090). This mRNA was used to check if the mRNA design spanning the protein CDS, RBS and the -10region of promoter was compatible to reduce noise from unwanted fluorophore. With our experiments, we can be sure that this antisense mRNA is very efficient in reduction of noise from Constitutively expressed eGFP. Teams working with BBa_J23100 BBa_B0034 and BBa_K4061002 (Constitutive promoter, Strong RBS and eGFP) could utilise this antisense molecule to suppress noise from the circuit. An additional use is if teams wish to characterise a promoter's activity, they could regulate this antisense mRNA under that promoter and observe the reduction in fluorescence intensity. This can be an indicator of promoter's function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None