Coding

Part:BBa_K3853017

Designed by: RuYi Ma   Group: iGEM21_CPU_CHINA   (2021-09-20)
Revision as of 00:12, 16 October 2021 by Lp-tiffany (Talk | contribs)


MnP(E74L)

Manganese peroxidase (MnP) is the key enzyme in our degrading system. In order to improve its catalyzing ability, we tried rational design. And according to the computational redesign results, 6 mutants were chosen and tested, including their relative enzyme activity and the effect of temperature/pH/organic solvents on them. MnP(E74L) is one of the most promising mutant of MnP. We use BBa_K3853060 to construct the expression system to express and purify the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None