Part:BBa_K3788002

6His-P20

BBa_K3788002 sequence is coding for P20 a chaperone protein in Bacillus thuringiensis. This chaperon have a histidin tag in Nter to allow to purified the protein P20 and allow the detection and specific identification of P20 by WesterBlot. The gene p20 was optimised for E. coli K12.

Expected size :

6his_p20 gene is 570bp

6His_P20 protein is 20 kDa

P20 chaperon:

Was discovered in B.thuringiensis, a p20 gene in operon with cry11Aa and <i<cyt1Aa</i>. Thus, the P20 protein was shown to be necessary for the formation of inclusion bodies of Cry11Aa and Cyt1Aa. P20 would allow a greater production of these toxins and allow their stability (Cyt1Aa would be protected from the proteolytic action before its maturation). The co-production of P20 and Cyt1Aa in E.coli would limit its toxic effects in the producer cell.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 154
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]