Regulatory

Part:BBa_K3930029

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-08)
Revision as of 13:07, 15 October 2021 by Ambre-Jousselin (Talk | contribs)


IPTG and theophylline inducible promoter Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

The promoter Ptrc-riboswitch E comes from the publication of Nakahira et al. 2013. It comprises a double regulation of the downstream gene expression. The first is a transcriptional regulation by the IPTG-inducible TRC promoter. The second regulation is post-transcriptional, as a theophylline-inducible aptamer sequesters the RBS essential for mRNA translation. This promoter has been originally described for control of gene expression in the cyanobacterium Synechococcus elongatus PCC 7942 .


References

  1. Nakahira Y, Ogawa A, Asano H, Oyama T, Tozawa Y. 2013. Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942. Plant and Cell Physiology. 54(10):1724–1735. doi:10.1093/pcp/pct115.
    2.



[edit]
Categories
Parameters
None