Regulatory

Part:BBa_K3930029

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-08)
Revision as of 09:45, 15 October 2021 by Ambre-Jousselin (Talk | contribs)


IPTG and theophylline inducible promoter Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

The promoter Ptrc-riboswitch E comes from the design of the publication Nakahira et al. 2013. It comprises a double regulation of the downstream gene expression. The first is a transcriptional regulation by the IPTG-inducible TRC promoter. The second regulation is post-transcriptional, as a theophylline-inducible aptamer sequesters the RBS essential for mRNA translation. This promoter has been originally described for cyanobacterium Synechococcus elongatus PCC 7942 control of gene expression.


References

  1. Nakahira Y, Ogawa A, Asano H, Oyama T, Tozawa Y. 2013. Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942. Plant and Cell Physiology. 54(10):1724–1735. doi:10.1093/pcp/pct115.
    2.



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