Coding

Part:BBa_K3822005

Designed by: Jiajia Li   Group: iGEM21_YiYe-China   (2021-09-30)
Revision as of 02:01, 15 October 2021 by Ljj (Talk | contribs) (→‎1.Construction of the plasmids)


miR196a-148a 2.0

miR196a-148a 2.0 is an improvement of Part BBa_K3160007. which could monitor the expression of miRNAs in gastric cancer cells

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Result

1.Construction of the plasmids

The miRNA “ sponge” method was introduced by several years ago as a means to create continuous miRNA loss of function in cell lines and transgenic organisms. More effective are sponges containing bulged sites that are mispaired opposite miRNA positions 9-12 (Ebert et al. 2007, Gentner et al. 2009), presumably because they form a more stable interaction with the miRNA. pEGFP-miR196a-148a 2.0(Part K3822005) was designed based on the principle of effective sponges containing bulged sites (Fig. 1). We compare pEGFP-miR196a-148a 2.0 with pEGFP-miR196a-148a 1.0 (Part K3160007,designed by a 2019 iGEM team) on monitoring the expression of miRNAs in gastric cancer cells. T--YiYe-China--Improve1.png

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Parameters
None