Coding
Part:BBa_K3930008
Designed by: Thomas Gaudin Group: iGEM21_Toulouse_INSA-UPS (2021-10-07)
Gene coding for the Linalool synthase
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 1000COMPATIBLE WITH RFC[1000]
Introduction
This sequence codes for the Linalool synthase, an enzyme that transform GPP into linalool, a molecule which smell like flower. The sequence derived from the Lavandula angustifolia genome. We take advantage of the publication of (Amiri et al. 2016).
There is already BBa_K3052002 on the registry for linalool synthase. This part is codon optimized for translation in E. coli, but ours is codon optimized for translation in S. cerevisiae.
References
- Amiri P, Shahpiri A, Asadollahi MA, Momenbeik F, Partow S. 2016. Metabolic engineering of Saccharomyces cerevisiae for linalool production. Biotechnol Lett. 38(3):503–508. doi:10.1007/s10529-015-2000-4.
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Categories
Parameters
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