Reporter

Part:BBa_E0051:Design

Designed by: Austin Che   Group: iGEM06_MIT   (2009-03-01)
Revision as of 19:18, 2 March 2009 by Austin (Talk | contribs)

lacZa.GFP fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 878


Design Notes

The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc. The start codon from GFP was removed.


Source

amino acids 1-60 from lacZ and amino acids 2-end of GFP (BBa_E0043)

References