Regulatory

Part:BBa_K3927002:Design

Designed by: Chew Chin Wei   Group: iGEM21_NUS_Singapore   (2021-09-29)
Revision as of 18:31, 14 October 2021 by CWChew (Talk | contribs) (→‎Source)

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3C120-CYC-LacO


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 203
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 185
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 203
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 203
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have added an additional nucleus localisation signal peptide at the 5' side of the coding sequence


Source

Synthetic part based on EL222 optogenetic system in E. coli.

References